Human Monoclonal Antibodies Methods and Protocols

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.