This updated and revised second edition acts as an introduction to the conceps and techniques of recombinant DNA research and their results. The book features 14 new chapters and 11 rewritten chapters and incorperates research published throughout 1991. The coverage of recombinant DNA centres largely on key experiments, with sections focusing on new developments in cloning, mutagenisis, and genetic engineering, plus the contribution of recombinant DNA technology to our understanding of gene function, biological processes and human genetics.